Cellular Cancer Therapy, part 11

CHAPTER 11 : Metabolism of Neoplastic Tissues

    Popp demonstrated that in the interaction between the bioreceptor and the carcinogen at least three processes should exist to produce the alteration called CANCER: the transfer of electric charge, the chemical reaction, and the transfer of energy.

    Mason, Hoffman, Lakik, Allison and Nash have demonstrated that the transfer of electrical charge is not very probable because the significant correlation between the transfer of charge of the molecules and their oncogenic activity has not been found, as for example happened with the indices of the transfer of electrical charge and the hallucinogenic properties of some drugs.

    It has been shown that the relation between the covalent bond of viral molecules and the cellular DNA or the intracytoplasmic proteins. The transfer of energy is the exchange of photons in any form. This connection is obvious in the case of the inducing of cancer by radiation. This radiation should have an energetic value of approximately 3 eV to induce cancer.

    The polycyclic hydrocarbons and some viral DITA molecules show Fermi resonances for p>- and a>- states in the range of approximately 3 - 4 eV, with a possible relation to oncogenetic activity. Besides this, Fermi resonances cause a specific alteration in the ordinary absorption and in the remission of UV photons in this range of critical energy. According to the latest reports and experiments that have been published, the existence of long wave UV biophotons has been shown. There are reasons to believe that these photons are important for the regulation of the development of the cell population and therefore for the inducing of cancer and its development.

    As a result of all this, the nucleic acids which become conducting after being excited with energy forms of greater than 3 eV become the focus of interest. It cannot be supposed that the DNA or RNA molecule represents a fluid stationary energy state, since this is the result of a weak quantization, in turn due to the interaction of the molecules with the cell environment. We could deduce, based on the regulated functions of cellular development and reproduction such as phases of differentiated cycles, transcription, self—duplication and mitosis that the controlled transfer of energy takes place with DNA and RNA.

    Because of their regular structure DNA or RNA can be mathematically represented in the following way:

(1a)  [missing]

(1b)  [missing]

(1a) represents vertical excitations which in turn are made up of horizontal excitations; according to (1b) and in general are not stable states of DNA.

    j represents the states of the paired bases that constitute superpositions of states of isolated molecules. N4 and N are for normalization; a is the vertical distance from the neighboring paired base, a is the angle of rotation in relation to the double strand of DNA, whose axis is shown in direction z. A system of such magnitude can be excited vertically and horizontally. There is a coupling between these two types of excitation. The coupling by the "moment" operator adds to the transfer of electrical charge between the stored paired bases. These states can decay if they are coupled by the moment angle operator in states of triplets of paired bases. The exact focus of the problem is very difficult to determine, especially because of the existence of stationary states cannot be presupposed. Due to the fact that macromolecules like DNA or RNA show properties that should be found among classic and "quantic" phenomena, the consideration of a classical model could have some advantages.

    It has been demonstrated that DNA can function and does function as a resonant circuit in which the DNA is the coil and the cell membranes act as the condensers. This circuit yields resonance energies that fluctuate between 2 and 6 eV. In the classical model mentioned above, the vertical charge transfer induces specific biophotons shaped by the solar UV rays. It has been proven that biophotons can be retransmitted by the DNA circuit greatly amplified when the nucleic acid is resonated by the action of osmotic influences of viral proteins or by triplet or single changes in energy state. The viral proteins change the cell action potential by altering the ratio of intracellular vs. extracellular sodium and intra vs. extracellular potassium.

Metabolism of the cancerous cell

    Unger postulated that malignant cells are different from normal cells in several important characteristics that are based on or influenced by the cell surface. From among these, the following seem more important: lack of inhibition by contact, alterated immunological behavior, and invasive development (production and release of enzymes that cause adjacent tissues to deteriorate).

Inhibition by contact

    Taking into consideration the behavior of normal cells and malignant ones in culture, it can be observed that before merging, both types of cells manifest a certain degree of motility and development. As soon as the merger takes place, the motility and development of the normal cells cease. This phenomenon is called inhibition by contact, and is lacking, to a varying degree, in malignant and embryonic cells. According to the state of our present knowledge, cellular proliferation is regulated by cell—to—cell contact. If this contact is missing, adenyllic—cyclase is inactivated, AMP is not formed, and the self—duplication of DNA is not repressed. Through functional contact, adenyllic—cyclase is activated, producing AMP which inhibits the synthesis of DNA.

Deficient immunological response

    The development of a tumor, like that of metastases depends, among other factors, on the antigenicity of the respective tumoral cells. Therefore, malignant cells from which some antigenetic determinants have been removed metastasize with great speed and intensity, while antigenetically intact cells do not metastasize.

Invasive development

    Tumoral cells "filter out" certain enzymes, like collagenase, an enzyme which depolymerizes collagen and contributes to invasive development. Fuddenberg has shown that the, production of hyaluronidase in some sarcomas and lymphomas just as in carcinomas of the mammary glands. This enzyme, like collagenase, depolymerizes collagen contributing to the "seeding" of the malignant cells.

The chemical nature of cell surfaces

    The basic structural characteristic of cell membranes is the double layer of lipids between which are sandwiched the protein molecules. The lipids as well as the proteins can transport carbohydrates as lateral chains and it is supposed that they are turned ‘towards the cell exterior. The carbohydrates constitute the principal structural determinants involved in the cell surface processes in mammals. The differences and the changes discovered between the surfaces of normal cells and malignant cells can be divided into two broad groups.

    The first refers to the observed changes in the surface gluco— proteins, and the second to the alternations in the composition of the gangliosides and to the differences in the agglutinating behavior of the cells in relation to the special vegetable glucoprotein group called lectins.

    Warren et al. at the National Institutes of Health have carried out an extensive series of experiments about the changes in the surfaces of glucoproteins when they become malignant. Originally, they found that a glucopeptide that contained fucose was significantly more present over the surface of cells affected by the polioma virus, in the murine viral sarcoma and in the tissues invaded by the Rous sarcoma. Through the use of a temperature sensitive mutation of the Rous sarcoma virus, it was shown that the change in the glucopeptide was controlled by the expression of the viral genome. The change in the glucopeptide that contained fucose was due to an added amount of scialic acid in the transformed material. The biosynthesis of the amino—sugars of the cell surface from glucose is shown in the following schematic description:

    The amino group is incorporated starting with glutamine or ammoniac in a condensation stage that takes the fructose—6—phosphate to glucoseamine—6—phosphate. The Acetyl-coenzyme A is the donor of the acetyl group in all of the stages of acetylization. As we will see below there are several reactions of phosphoryllation and desphosphoryllation involved. N—acetyl— glactosamine is formed as well as its activated derivative UDP—N—acetylgalactosamine by a 4-epimerase that comes from the UDP-N-acetylglucosamine. N—acetylmanosamine is also formed from UDP—N—acetylglucosamine through the action of a 2—epimerase that also cuts through the residual nucleosidopyrophosphate. With hexose and hexosamine sugars, the enzymes that transfer them to their individual receptors have habitually been called glucosiltransferases. Their substrates are activated sugars; that is, nucleotide—sugars. These are: UDP—N—acetylglucosamine; UDP—N—acetylgalactosamine; UDP-N-glucosamine, UDP—glucose, UDP—galactose, GDP—manose and GDP-fucose, GDP—fucose being synthesized from GDP—manose by reduction and isomerization.

    Radical changes have been observed in the composition of the gangliosides of the cell surfaces concurrently with the malignant transformation, there being a shortening of the lateral glucosile chain in the gangliosides of the cell surfaces in malignant cells. There is no gangliosido—glucosil— transferase in malignant cells.

Fig. 11.1 is a diagram of ‘the metabolism of the amino—sugars of the cell surface.

CHAPTER 12 : Laboratory Diagnosis of Cancer: The Oncodiagnosticator

[Note: The method described below was used in the past, and may be revived in the future.  But  it is still experimental, and we do not know of any doctor or lab who uses it today.  A small preliminary study  by SGA MD, at McGill University in 1975, found no predictive value.  But the method has not, to my knowledge, been tested in any other laboratory. -- IPTQ.org]

    Every patient who comes to our clinic is tested for cancer with the Oncodiagnosticator.

METHOD

    Ten cc of blood are taken (see Fig 1—b), put into a test tube (fig. 2—b,) and put into a centrifuge.

    After three minutes in the centrifuge at 3000 rpm, 3 ml of blood serum are taken and put in a small parchment bag (semipermeable membrane) about 10 x 10 cm (fig. 4-b). This little bag is put into a 100 ml graduated cylinder (fig. 5—b,) with 40 ml of distilled water — the level of serum in the bag should be lower than the level of the water in the recipient. (se vacían en un vaso y se coloca en aparato??) (see fig. 5 bis)

    Two thin (1.5 mm) copper wires are connected to the apparatus (fig. 6-b,), put into the water, one on either side of the recipient, and the parchment bag with the serum is put into the recipient as well (fig. 7—b). The voltage on the Oncodiagnosticator is set at 32 volts (fig. 8—b), it is turned on and left for two hours. Afterwards it is disconnected and the final pH is read (fig. 9—b,). The serum from the little bag (fig. 10—b) is transferred to a glass test tube (fig. 11—b, 12—b, 13—b) so that its color can be observed against a sunlit background and a color scale. The Oncodiagnosticator is an instrument made up of a voltmeter, [a power supply,] and an ammeter.

INTERPRETATION

    This inexpensive, simple test yields very important information about whether the patient has a malignant process in his body, whether it is plainly developing, hidden or if it is only a predisposition.

    In a negative test, the serum retains its characteristic straw—yellow color in most individuals.

    In a positive test, the serum acquires a purple or violet color in any of the possible shades. The intensity of the coloring is directly proportional to the degree of malignancy of the process.

    Some patients will show a cancer—negative reaction that is a dark brown coffee color or even other colors. Dark brown indicates a state of extreme toxicity in the individual. When this color appears with purple (which can be observed better if the sample is left overnight — the brown decants and the purple appears in the upper layers, sometimes this is even observable immediately), this indicates a very bad prognosis for the patient’s life.

    The Oncodiagnosticator can also serve to prove that a patient has been cured or show the degree of malignancy (as a function of the intensity of the violet coloring acquired by the serum).

    When the oncodiagnostic method produces a positive result and the patient shows no clinical manifestation of malignant neoplasia, experience has shown us after 13 years that the patient will not get better unless efficient cancer therapy is given (i.e., Donatian Therapy).

    Patients who have been cured through the use of Donatian Therapy for malignant neoplasias have trimesterly follow—up exams consisting of a simple and quick oncodiagnostic examination of their serum.

    The change in color in the oncodiagnostic test is due to the presence of abnormal proteins and nucleoproteins with abnormal DNA and RNAs that have a higher molecular weight in the serum of cancer patients. These neucleoproteins synthesized by cancerous tissues combine with the copper of the electrodes and because they contain a lot of scialic acid they form copper scialates which are salts that become purple when they oxidize.

    We have observed that in cancerous patients that show metastasis, the color has always been purple and the milliamperage has never gone above 58 milliamps.

    In sum, the Oncodiagnosticator is an instrument basically made up of a voltmeter and an ammeter, with copper wire electrodes (that should be changed after every fourth test), used in the diagnosis of cancer.

THE DIAGNOSTIC MECHANISM

    The electric current (32 volts) forms a dipole 300 times more intense than the electric dipole of the cell; therefore, the redox potential increases considerably. In this electric environment, the proteins (nucleoproteins with abundant quantities of scialic acid, see Chapter Two) combine with the Cu+ ions released by the electric field, forming Cupric scialoproteinates which acquire a purple color according to their quantity.

CONCLUSIONS

    The patient will have shown a positive cancer response in this test if, two hours after the 3 ml sample of blood has been centrifuged at 3000 rpm for three minutes, the sample shows:

1. a violet or purple color.

2. the milliamperage of the apparatus is above 70 miliamps.

    The increase in intensity of the current in the cancerous patient is due to the increased potential oxide reduction through the fixing of scialoproteins to the copper ion of the electrodes, which does not occur in non—cancerous patients.

    Obviously the temperature of the liquid (distilled water) will rise, due to the increased redox potential and the elevation of the milliamperage, to as high as 82°C (see fig. 1k—b).

DESCRIPTION OF THE ONCODIAGNOSTICATOR AND ITS USE

    This instrument permits, as its name indicates, the diagnosis of cancer in any patient, to confirm suspicions in those who might have it and as a preventive examination in healthy individuals.

    The construction of the instrument uses physical and chemical elements:

    Physical elements: voltmeter, ammmeter, interchangeable copper electrodes, glass recipient, and parchment—paper membrane bag.

    Chemical elements: colorless catalyzer: distilled water.

    Scientific basis: the voltmeter is used to measure the difference in potential no higher or lower than 32 volts so as to guarantee the reaction. The milliammeter measures the electric current due to the migration of the ions, from the cathode to the anode or vice versa.

    The copper electrodes have the peculiarity of transmitting the current by being very good conductors of electricity, besides which the metal combines with other elements, yielding copper salts. Glass does not interfere in the reactions, which is why the recipient is made of this material.

    To mimic as close as possible to the characteristics of the cell membrane, a semipermeable membrane of parchment is used which only permits the passage of certain substances.

    The serum and not the plasma is used because of its characteristics: it is a transparent yellow liquid with minerals, lipids, carbohydrates and proteins. All of these elements, and especially the proteins, are what permit the change in color at the end of the reaction, and which serve as the basis for the early and exact diagnosis of the supposed cancer patient. Besides making precise the situation and degree of development of the cancer, it also indicates the predisposition for contracting it soon, that is, the resulting color determines the absence (organic equilibrium), the propensity (organic terrain tending toward bio—physico—chemical disequilibrium), or the gravity of the cancer (organism with manifest disequilibrium which has fostered the development of the disease).

    Nevertheless, the lipids, carbohydrates, and proteins, which also have electrical charge (they are polar substances), do not go over the potential difference of 32 volts when they are amplified by the cell condensers (basically the membrane and the ribosomes).

    The ions of the cell liquids, upon being stimulated by the current, are released from the serum and pass through the pores of the parchment membrane (while the lipids, proteins and carbohydrates cannot). Thus the copper salts are formed, in relation with the metabolic equilibrium or disequilibrium extant in the individual, and these copper salts decant to the bottom of the recipient.

    The reagent does not intervene directly in the reaction, since it is outside of the membrane and its function is to demonstrate the reactions.

    The ionic changes of the serum, originated by the passage of electric current as a function of the type of elements that it contains, cause the color. Therefore, the minerals that the serum contained can be found in the external liquid. In the internal liquid the lipids, proteins, and carbohydrates are found, the proteins being the ones that give the serum its distinctive color according to the state of the patient.

    The reaction takes two hours at 32 volts, as mentioned above. At the end of this period the contents of the internal liquid are emptied into a flask identified with the patient’s name.

    To summarize, the oncodiagnosticator, together with Donatian Therapy, becomes the most efficient weapon for the prevention of cancer or for its treatment.

    When the serum of a donor is to be used for the preparation of a vaccine, the donor should first be studied with the Oncodiagnosticator to see if his/her serum can be usable in the patient who will receive the vaccine. Because if the Oncodiagnosticator indicates propensity or asymptomatic cancer, the serum of the donor cannot be used and he himself should also begin treatment.

    In the administration of Hemo—immunoglobin the donor should also be examined beforehand with the Oncodiagnosticator. Therefore, we suggest that it be put into use at every medical institution.

Photo captions      [Photos will be included, once they are found.]

Fig. 1— 7—10 cc of blood are taken for the oncodiagnostic test.

Fig. 2— The blood is put into a test tube.

Fig. 3— The test tube is put into the centrifuge to separate the serum from the coagulate.

Fig. 4— The serum is put into the parchment membrane bag.

Fig. 5— 40 cc of distilled water are measured.

Fig. 5— The recipient is put into the holder.

Fig. 6— The electrodes are put in place.

Fig. 7— The parchment bag with the extracted serum is put into the recipient.

Fig. 8— The apparatus is turned on and kept at 32 volts for two hours.

Fig. 9— The pH of the external liquid is measured at the end of the reaction.

Fig. 10— The serum in the bag is emptied into a glass container.

Fig. 11— The color of the liquid is examined; if it is yellow as in the picture, then the result is negative.

Fig. 12— If the color is violet, then the result is positive.

Fig. 13— A series of test tubes: the three first (from the left) are negative, and the next three are positive. The intensity of the purple will show if there is only a propensity, or the disease itself, even if there are no manifest signs, symptoms or laboratory results. A very intense violet appears when both clinically and in the laboratory evidence of the disease appears. It is very important to note that due to differences in alimentation, customs, habits, and environment the resulting violet color varies in the shades it can manifest. This we found when performing tests at McGill University in Montreal; the scale of colors was very different from that obtained working with patients from Mexico City. Dr. Thomas Tallberg, of the University of Finland in Helsinki, has reported similar, variations. These variations, we would like to stress, are due only to differences in alimentation and environment.

Fig. 14— On the upper left, the voltmeter. Below, the switch for adjusting the voltage. In the middle, the on/off switch. On the upper right, the ammeter, with the electrodes and recipient holder. In the middle, an optional instrument to record fluctuations in milliamperage during the reaction.